Effect of extended cold ischemia time on glucose metabolism in liver grafts: experimental study in pigs
- 27 March 2007
- journal article
- Published by Springer Nature in Journal of Hepato-Biliary-Pancreatic Surgery
- Vol. 14 (2), 183-188
- https://doi.org/10.1007/s00534-006-1127-z
Abstract
Recovery of normal carbohydrate metabolism in the liver after transplantation is highly important. The aim of the present study was to evaluate how short and long cold ischemia (CI) time followed by warm ischemia (WI) impact intrahepatic glucose metabolism in a pig liver transplantation model. Twenty-six animals were divided into two transplantation groups: group I with a liver ischemia time of 5 h (n = 6), and group II with 15 h of liver ischemia (n = 7). Intrahepatic microdialysis samples were collected throughout the experiment at 20-min intervals, during the donor operation, cold preservation, liver implantation, and liver reperfusion in the recipient. Glucose, lactate, and pyruvate concentrations were analyzed and the lactate/pyruvate ratio (L/Pr) was calculated. There were no changes in glucose levels during CI. However, during WI, glucose and lactate increased and the increase was significantly higher in the group with longer CI (P < 0.01). The L/Pr increased at the beginning of CI but accelerated to increase during WI in both groups, with significantly prolonged and higher levels in the group with longer CI (P < 0.01). Extended CI results in increased intrahepatic glycogenolysis, delayed restoration of aerobic glycolysis, and prolonged anaerobic glycolysis shortly after reperfusion. Improvements in glycogen protection and faster restoration of aerobic metabolism during preservation and reperfusion time seem to be necessary in order to improve liver preservation protocols per se.Keywords
This publication has 19 references indexed in Scilit:
- Review article: improved preservation of the liver for transplantationAlimentary Pharmacology & Therapeutics, 2007
- Hepatic cell membrane damage during cold preservation sensitizes liver grafts to rewarming injuryJournal of Hepato-Biliary-Pancreatic Surgery, 2003
- Clinical experience in continuous graft monitoring with microdialysis early after liver transplantationBritish Journal of Surgery, 2002
- Metabolic changes in the liver graft monitored continuously with microdialysis during liver transplantation in a pig modelLiver Transplantation, 2002
- Glycolysis and Energy Metabolism in Rat Liver during Warm and Cold Ischemia: Evidence of an Activation of the Regulatory Enzyme PhosphofructokinaseCryobiology, 1994
- Microdialysis of adipose tissueJournal of Internal Medicine, 1991
- Liver transplantation: Results, advances and problemsJournal of Gastroenterology and Hepatology, 1990
- PANCREATIC HORMONAL RESPONSES TO HYPERGLYCEMIA DURING ORTHOTOPIC LIVER TRANSPLANTATION IN MANTransplantation, 1989
- RELIABLE INDICES FOR THE DETERMINATION OF VIABILITY OF GRAFTED LIVER IMMEDIATELY AFTER ORTHOTOPIC TRANSPLANTATION BILE FLOW RATE AND CELLULAR ADENOSINE TRIPHOSPHATE LEVELTransplantation, 1988
- Hepatic transplantation survival: Correlation with adenine nucleotide level in donor liverHepatology, 1988