AN EVALUATION OF THE FRANK METHOD FOR THE DETERMINATION OF PROLAN (GONADOTROPIC PRINCIPLE) IN THE URINE OF NONPREGNANT WOMEN1

Abstract

292 detns. were made on the urine of 248 [female][female]. Of these 235 presented a clinical picture which suggested abnormal gonadotropic function of the ant. lobe. 13 apparently normal [female][female] were used as controls. The clinical diagnosis in each case was made before the prolan detn. was reported. The amt. of prolan found in the urine helped to substantiate the diagnosis. Immature mice were used, and the uteri and ovaries weighed and the ovaries sectioned and stained. Instead of 60 cc. of urine, 400 cc. was used, and in the final amt. for biologic assay 2 cc. represented 100 cc. urine.