Rapid detection of chloramphenicol resistance in Haemophilus influenzae
- 1 August 1981
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 20 (2), 168-170
- https://doi.org/10.1128/aac.20.2.168
Abstract
We compared a rigid (1-h) screening method for the detection of chloramphenicol acetyltransferase (CAT) activity with the standard spectrophotometric CAT assay to determine whether CAT-mediated chloramphenicol resistance in Haemophilus influenzae could be determined upon primary isolation. Of 58 H. influenzae cell sonicates, 28 had detectable CAT activity when the chloramphenicol-dependent production of free coenzyme A from acetyl coenzyme A was measured spectrophotometrically (standard method). These 28 strains were identified as producing CAT by the rapid method which uses lysed cell suspensions and a color change to detect CAT. The remaining 30 strains did not have CAT activity detectable by either method. This 1-h test for CAT should prove to be useful for the early presumptive identification of chloramphenicol resistance in H. influenzae.This publication has 8 references indexed in Scilit:
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