Identification of asparagine-linked oligosaccharides involved in tumor cell adhesion to laminin and type IV collagen.
Open Access
- 1 October 1984
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 99 (4), 1416-1423
- https://doi.org/10.1083/jcb.99.4.1416
Abstract
MDW4, a wheat germ agglutinin-resistant nonmetastatic mutant of the highly metastatic murine tumor cell line called MDAY-D2 has previously been shown to attach to fibronectin and type IV collagen, whereas MDAY-D2 and phenotypic revertants of MDW4 attached poorly to these substrates. The increased adhesiveness of the mutant cells appeared to be closely related to a lesion in cell surface carbohydrate structures. In an effort to identify the carbohydrates involved in cell attachment, glycopeptides isolated from mutant and wild-type cells as well as from purified glycoproteins were tested for their ability to inhibit the attachment of MDW4 cells to plastic surfaces coated with fibronectin, laminin, or type IV collagen. The addition of mannose-terminating glycopeptide to the adhesion assay inhibited MDW4 cell attachment to type IV collagen. In contrast, a sialylated poly N-acetyllactosamine-containing glycopeptide, isolated from wheat germ agglutinin-sensitive MDAY-D2 cells but absent in MDW4 cells, inhibited MDW4 attachment to laminin. None of the glycopeptides used in this study inhibited attachment of MDW4 cells to fibronectin-coated plastic. Peptide N-glycosidase treatment of the cells to remove surface asparagine-linked oligosaccharides inhibited MDW4 adhesion to type IV collagen, but not to laminin, and the same treatment of the wheat germ agglutinin-sensitive cells enhanced attachment to laminin. Tumor cell attachment to, and detachment from, the sublaminal matrix protein laminin and type IV collagen are thought to be important events in the metastatic process. Our results indicate that tumor cell attachment to these proteins may be partially modulated by the expression of specific oligosaccharide structures associated with the cell surface.Keywords
This publication has 47 references indexed in Scilit:
- Asparagine-linked oligosaccharides in murine tumor cells: comparison of a WGA-resistant (WGAr) nonmetastatic mutant and a related WGA-sensitive (WGAs) metastatic line.The Journal of cell biology, 1984
- Complex carbohydrates of the extracellular matrix structures, interactions and biological rolesBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1982
- Structure, biosynthesis and functions of glycoprotein glycansCellular and Molecular Life Sciences, 1982
- The role of fibronectin in adhesion of metastatic melanoma cells to endothelial cells and their basal laminaExperimental Cell Research, 1981
- Metastatic Potential Is Positively Correlated with Cell Surface Sialylation of Cultured Murine Tumor Cell LinesScience, 1981
- Studies on cell adhesion and recognition. I. Extent and specificity of cell adhesion triggered by carbohydrate-reactive proteins (glycosidases and lectins) and by fibronectin.The Journal of cell biology, 1981
- Cell surface carbohydrates of embryonal carcinoma cells: Polysaccharidic side chains of F9 antigens and of receptors to two lectins, FBP and PNACell, 1979
- Teratocarcinoma stem cells have a cell surface carbohydrate-binding component implicated in cell-cell adhesionCell, 1979
- Fibronectin–plasma membrane interactions in the adhesion and spreading of hamster fibroblastsNature, 1978
- Non-metastasising variants selected from metastasising melanoma cellsNature, 1977