Identification of domains of the v-crk oncogene product sufficient for association with phosphotyrosine-containing proteins.
Open Access
- 1 March 1991
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 11 (3), 1607-1613
- https://doi.org/10.1128/mcb.11.3.1607
Abstract
The oncogene product of the avian sarcoma virus CT10, P47gag-crk, contains the SH2, SH29, and SH3 domains and binds proteins in a phosphotyrosine (ptyr)-dependent manner. In this study, we have determined the region of P47gag-crk essential for binding to ptyr-containing proteins. Mutant P47gag-crk proteins expressed in Escherichia coli that have the intact SH2 and SH29 regions retained the capacity to bind ptyr-containing proteins obtained from cells transformed by crk and src. The deletion of SH2 resulted in the loss of binding activity. Other mutants that have altered SH2 or SH29 bound few, if any, of the ptyr-containing proteins. Those mutants that bound ptyr-containing proteins associated with tyrosine kinase activity. We also found that polypeptides containing SH2, SH29, and SH3 of p60v-src and p60c-src associated with ptyr-containing proteins from crk-transformed cells. Thus, the SH2 and SH29 domains of P47gag-crk are responsible for their binding to ptyr-containing proteins. ImagesKeywords
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