Effects of Flow Rate and Eluant Composition on the High Performance Liquid Chromatography of Proteins
- 1 January 1980
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 3 (9), 1373-1383
- https://doi.org/10.1080/01483918008062783
Abstract
The influence of eluant flow rate on efficiency in reverse-phase and ion-exchange high performance liquid chromatography of proteins was studied. Both isocratic and gradient elution led to the same conclusions. The use of flow rates much lower than those generally employed was important for the efficient separation of large and small proteins. Several eluant systems were examined to determine the effects these had on the efficiency of the reverse-phase separation of proteins.Keywords
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