OBSERVATIONS ON THE ETIOLOGIC RELATIONSHIP OF ACHYLIA GASTRICA TO PERNICIOUS ANEMIA. VIII. FURTHER STUDIES OF THE PROTEOLYTIC ACTIVITY OF NORMAL HUMAN GASTRIC JUICE IN VITRO; AND THE LIMITATIONS OF THE METHOD IN PERNICIOUS ANEMIA 1

Abstract

The so-called intrinsic factor in gastric juice which leads to remission in pernicious anemia was correlated with the proteolytic enzyme of the gastric juice in 2 additional respects. Adsorption by Lloyd''s reagent completely removed both the clinical activity and the proteolytic power of gastric juice. Dialysis of gastric juice for periods of 3 to 5 days did not impair either the activity of the gastric juice in patients with pernicious anemia or the proteolytic activity of this material against casein. The use of the proteolytic activity of gastric juice as a means of diagnosing pernicious anemia was found unsatisfactory because of the presence of large amts. of tryptic-like enzymes in the gastric juice of patients with pernicious anemia.