Nature and Development of Membrane Systems in Food Vacuoles of Cellular Slime Molds Predatory upon Bacteria
- 1 September 1965
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 90 (3), 755-+
- https://doi.org/10.1128/jb.90.3.755-765.1965
Abstract
During the digestion of bacteria by the myxamoebae of cellular slime molds, systems of concentric lamellae begin to appear within the food vacuoles. Each constituent lamella is a unit membrane of 75 to 85 A thickness. A study of these lamellae in Dictyostelium discoideum and Polysphondylium pallidum reveals that most of them do not represent original membranes of the ingested bacteria but are formed mainly in two ways. (i) After swelling and partial digestion of the bacteria, the first membranes appear adjacent to preexisting membranes, e. g., the membrane lining the food vacuole and the cytoplasmic membrane surrounding the bacterium. Progressive addition of lamellae leads to the formation of the systems of concentric lamellae. (ii) After digestion of the bacteria has proceeded to a high degree, the concentric lamellae are formed spontaneously from clouds of amorphous material through condensation and orientation of precursor material. The study shows that, in biological systems, unit membranes may be formed from amorphous material through template action of pre-existing membranes, and does not necessarily involve fusion of membrane-bound vesicles.This publication has 23 references indexed in Scilit:
- Fate of the cell wall of Salmonella typhimurium upon ingestion by the cellular slime mold: Polysphondylium pallidumBiochemical and Biophysical Research Communications, 1965
- Electron Microscope Observations on Spore Formation in the True Slime Mold Didymium nigripesThe Journal of Protozoology, 1964
- Electron microscope studies of the fertilized egg and the two-cell stage of Mytilus edulisJournal of Ultrastructure Research, 1964
- ELECTRON MICROSCOPE STUDIES ON THE ORIGIN OF ANNULATE LAMELLAE IN OOCYTES OF NECTURUS The Journal of cell biology, 1963
- Growth of the Cellular Slime Mold Polysphondylium pallidum in a Simple Nutrient MediumScience, 1963
- Further studies in the ultrastructure of AcrasiaeExperimental Cell Research, 1961
- Electron Microscopy of Solitary and Aggregated Slime Mould CellsThe Journal of cell biology, 1960
- The ultrastructure of cells and cellulose membranes in AcrasiaeExperimental Cell Research, 1959
- ELECTRON MICROSCOPY OF BASOPHILIC STRUCTURES OF SOME INVERTEBRATE OOCYTESThe Journal of cell biology, 1956
- ELECTRON MICROSCOPY OF BASOPHILIC STRUCTURES OF SOME INVERTEBRATE OOCYTESThe Journal of cell biology, 1956