Mechanisms Regulating the Renal Transport of p-Aminohippurate

Abstract

The uptake of PAH was studied by the method of Cross and Taggart. Secretion was studied in two ways: a) measuring the movement of PAH into the ambient medium from renal slices which had been preloaded with PAH by the intraperitoneal injection of this compound into rats 40 minutes before killing, and b) measuring the uptake of PAH by guinea-pig renal slices, its rate of acetylation, and the movement of the newly-formed paraacetylaminohippurate (PAAH) from the tissue cells into the medium. The velocity of secretion is approximately 5–7 times that of uptake. Uptake is inhibited by 2,4-dinitrophenol, cyanide and Pentothal, while secretion is not. Pentothal also blocks the stimulatory effect of acetate on the uptake of PAH. The interpretation of the effects of Pentothal, with regard to the mechanism of the stimulatory effect of acetate on uptake, is discussed.