Functional implications of interleukin‐1β based on the three‐dimensional structure
- 1 January 1992
- journal article
- review article
- Published by Wiley in Proteins-Structure Function and Bioinformatics
- Vol. 12 (1), 10-23
- https://doi.org/10.1002/prot.340120103
Abstract
The molecular structure of interleukin‐1β, a hormone‐like cytokine with roles in several disease processes, has been determined at 2.0 Å resolution and refined to a crystallographic R‐factor of 0.19. The frame‐work of this molecule consists of 12 antiparallel β‐strands exhibiting pseudo‐3‐fold symmetry. Six of the strands make up a β‐barrel with polar residues concentrated at either end. Analysis of the three‐dimensional structure, together with results from site‐directed mutagenesis and biochemical and immunological studies, suggest that the core of the β‐barrel plays an important functional role. A large patch of charged residues on one end of the barrel is proposed as the binding surface with which IL‐1 interacts with its receptor.Keywords
This publication has 41 references indexed in Scilit:
- Crystal structure of recombinant human interleukin-1β at 2·0 Å resolutionJournal of Molecular Biology, 1989
- Site-specific mutagenesis of the human interleukin-1β gene: Structure-function analysis of the cysteine residuesBiochemical and Biophysical Research Communications, 1988
- Muteins of human interleukin‐1 that show enhanced bioactivitiesFEBS Letters, 1987
- Synthetic C-terminal peptide of IL-1 functions as a binding domain as well as an antagonist for the IL-1 receptorBiochemical and Biophysical Research Communications, 1987
- Point mutations of human interleukin‐1 with decreased receptor binding affinityFEBS Letters, 1986
- A 1H‐NMR study of human interleukin‐1βEuropean Journal of Biochemistry, 1986
- There is more than one interleukin 1Immunology Today, 1986
- Dictionary of protein secondary structure: Pattern recognition of hydrogen‐bonded and geometrical featuresBiopolymers, 1983
- The protein data bank: A computer-based archival file for macromolecular structuresJournal of Molecular Biology, 1977
- The interpretation of protein structures: Estimation of static accessibilityJournal of Molecular Biology, 1971