Antibody affinity measurements
- 1 June 1990
- journal article
- review article
- Published by Wiley in Journal of Molecular Recognition
- Vol. 3 (3), 108-116
- https://doi.org/10.1002/jmr.300030304
Abstract
The use of antibodies in immunoaffinity separations represents one of the most specific methods for purifying substances of biological interest. Since the binding affinity of antibody greatly influences its behavior in such separations, it is often important to know the value of the antibody affinity expressed as an equilibrium constant K. The present review discussed the equations used in the quantitative analysis of antigen/antibody interactions and describes currently used experimental methods for measuring K values. Advantages and shortcomings of the solution phase and solid phase approaches used for measuring antibody affinity are discussed.Keywords
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