Bovine γ‐Aminobutyric AcidA Receptor Sequence‐Specific Antibodies: Identification of Two Epitopes Which Are Recognised in Both Native and Denatured γ‐Aminobutyric AcidA Receptors

Abstract

Polyclonal antibodies have been raised against synthetic peptides whose sequences correspond to the N-terminal 15 amino acids and the C-terminal 17 amino acids of the bovine .gamma.-aminobutyric acidA (GABAA) receptor .alpha.1 subunit. These antibodies were shown to react with the denatured GABAA receptor .alpha. subunit, Mr 53,000, in Western blots with both purified receptor and brain membranes as antigens. Also, both antibodies recognised both the purified and detergent-solubilised GABAA receptor as demonstrated by dose-dependent specific immunoprecipitation of the GABA and benzodiazepine binding sites from solution. Evidence is also presented to show brain-regional distribution of the expression of the .alpha.1 subunit.