A New Enzyme, β-Glucosidase, in Honey

Abstract
Summary The presence of a new enzyme, β-glucosidase, was shown in significant amount in all 11 honeys tested. Optimal conditions for activity over a 20-min period, using 0·2-Mp-nitrophenyl-β-D-glucopyranoside (β-PNPG) as a substrate, were pH 4·2 and temperature 60°C. Results of tests performed directly on untreated honey were similar to those obtained with dialysed honey.

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