H+‐translocating ATPase and Na+/H+ antiport activities in the plasma membrane of the marine alga Platymonas viridis
- 14 September 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 309 (3), 333-336
- https://doi.org/10.1016/0014-5793(92)80801-m
Abstract
Proton transport by the vanadate-sensitive ATPase in plasma membrane (PM) vesicles from the marine unicellular microalga Platymonas viridis was investigated. The ATP-dependent generation of ΔpH across the membranes of PM vesicles was followed by the changes in the absorbance of the aminoacridine probe. Acridine orange, Na+ caused the decay of ΔpH generated by the ATPase, the rate of the decay being dependent on the concentrations of Na+ added. The phenomenon was specific for Na+. Amiloride inhibited Na+-dependent ΔpH decay. The experiments support the idea of a Na+-extruding mechanism (H+-translocating ATPase plus Na+/H+ antiporter) operating in the PM of marine alga Pl. viridisKeywords
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