Glutamate dehydrogenase activity in soybean, and the effect on it of amino acids and growth substances

Abstract

Glutamate dehydrogenase (GDH) is largely particulate in soybean, and its specific activity is much higher in roots than in leaflets. The specific activity of GDH in soybean callus is considerably lowered by glycine and leucine and is markedly increased by glutamate, tyrosine, phenylalanine, and especially serine. Increasing concentrations of indoleacetic acid increase the specific activity of GDH in callus far more than they increase either fresh weight or specific activity of malate dehydrogenase. Increasing kinetin also causes an increase in the specific activity of GDH, but mainly at low indoleacetic acid concentrations.