Transient Transfection and Expression in the Obligate Intracellular Parasite Toxoplasma gondii
- 16 April 1993
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 260 (5106), 349-352
- https://doi.org/10.1126/science.8469986
Abstract
Toxoplasma gondii is a protozoan pathogen that produces severe disease in humans and animals. This obligate intracellular parasite provides an excellent model for the study of how such pathogens are able to invade, survive, and replicate intracellularly. DNA encoding chloramphenicol acetyltransferase was introduced into T. gondii and transiently expressed with the use of three vectors based on different Toxoplasma genes. The ability to introduce genes and have them efficiently and faithfully expressed is an essential tool for understanding the structure-function relation of genes and their products.Keywords
This publication has 17 references indexed in Scilit:
- Synthesis and secretion of proteins by released malarial parasitesMolecular and Biochemical Parasitology, 1992
- A Toxoplasma gondii rhoptry protein associated with host cell penetration has unusual charge asymmetryMolecular and Biochemical Parasitology, 1992
- Stable DNA transfection of a wide range of trypanosomatidsMolecular and Biochemical Parasitology, 1991
- Homologous recombination in Leishmania enriettii.Proceedings of the National Academy of Sciences of the United States of America, 1991
- Homologous Recombination and Stable Transfection in the Parasitic Protozoan Trypanosoma bruceiScience, 1990
- Development of a stable Leishmania expression vector and application to the study of parasite surface antigen genes.Proceedings of the National Academy of Sciences, 1990
- Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene.Proceedings of the National Academy of Sciences, 1989
- Expression of a Bacterial Gene in a Trypanosomatid ProtozoanScience, 1989
- The α- and β-tubulins of Toxoplasma gondii are encoded by single copy genes containing multiple intronsMolecular and Biochemical Parasitology, 1988
- Isolation and characterization of a monoclonal anti‐P30 antibody resistant mutant of Toxoplasma gondiiParasite Immunology, 1987