Transfection of Leishmania enriettii and expression of chloramphenicol acetyltransferase gene.
- 1 December 1989
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 86 (23), 9119-9123
- https://doi.org/10.1073/pnas.86.23.9119
Abstract
We report a transient expression transfection system in Leishmania enrietti. A hybrid gene containing an intergenic region of the .alpha.-tubulin cluster and the bacterial chloramphenicol acetyltransferase (CAT; EC 2.3.1.28) gene is expressed after transfection of L. enriettii with the hybrid plasmid. The expression of the CAT gene is dependent on the presence of sequences from the .alpha.-tubulin gene. The hybrid gene is also active in Leishmania braziliensis and Leishmania major.This publication has 36 references indexed in Scilit:
- Trans splicing involves a novel form of small nuclear ribonucleoprotein particlesNature, 1988
- Transcriptional mapping of Leishmania enriettii tubulin mRNAsMolecular and Biochemical Parasitology, 1986
- Chromosome location of four genes in LeishmaniaMolecular and Biochemical Parasitology, 1986
- Identification of a novel Y branch structure as an intermediate in trypanosome mRNA processing: Evidence for Trans splicingCell, 1986
- The 35-Nucleotide Spliced Leader Sequence Is Common to All Trypanosome Messenger RNA'sScience, 1986
- DISCONTINUOUS TRANSCRIPTION AND ANTIGENIC VARIATION IN TRYPANOSOMESAnnual Review of Biochemistry, 1986
- Structure of mRNA encoded by tubulin genes in Leishmania enriettiiMolecular and Biochemical Parasitology, 1985
- Apparent discontinuous transcription of Trypanosoma brucei variant surface antigen genesNature, 1984
- Isolation and characterization of an α-tubulin gene from Leishmania enriettiiMolecular and Biochemical Parasitology, 1983
- Tubulin genes are tandemly linked and clustered in the genome of trypanosoma bruceiCell, 1983