Proteomic analysis of the heat shock response inSynechocystis PCC6803 and a thermally tolerant knockout strain lacking the histidine kinase 34 gene
- 1 February 2006
- journal article
- research article
- Published by Wiley in Proteomics
- Vol. 6 (3), 845-864
- https://doi.org/10.1002/pmic.200500196
Abstract
Proteomic analysis of the heat shock response of wild type and a mutant of the histidine kinase 34 gene (Δhik34), which shows increased thermal tolerance, has been performed in the cyanobacterium Synechocystis sp. PCC6803. In vivo radioactive labelling demonstrates that major proteomic changes occur within 1 h of heat shock. 2-D DIGE and MS have been used to quantify changes in specific proteins following heat shock in the wild type and the mutant. Over 100 spots, corresponding to 65 different proteins alter following heat shock. Changes occur not only in the classical heat shock proteins but also in the protein biosynthetic machinery, amino acid biosynthetic enzymes, components of the light and dark acts of photosynthesis and energy metabolism. The Δhik34 cells have elevated levels of heat shock proteins under both non-heat shock and heat shock conditions, in comparison to the wild type, consistent with Hik34, or a down stream component, being a negative regulator of heat shock-responsive genes.Keywords
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