CHROMOSOMAL LOCALIZATION OF THE HUMAN PLACENTAL LACTOGEN-GROWTH HORMONE GENE-CLUSTER TO 17Q22-24
- 1 January 1982
- journal article
- research article
- Vol. 34 (2), 227-234
Abstract
Recombinant plasmid HCS-pBR322 containing a 550-base-pair (bp) insert of c[complementary]DNA to human placental lactogen (hPL) mRNA was 3H-labeled by nick translation and hybridized in situ to human chromosome preparations in the presence of 10% dextran sulfate. A high percentage of cells (80%) were found to exhibit label on the distal end of the long arm of chromosome 17. Ag grains on this region constituted 25.5% of all labeled sites, allowing assignment of the hPL and growth hormone (hGH) genes, which have over 90% nucleotide homology in their coding sequences, to 17q22-24. A gene copy number experimental showed that both genes are present in .apprx. 3 copies/haploid genome.This publication has 24 references indexed in Scilit:
- The Prolactin Gene Is Located on Chromosome 6 iIn HumansScience, 1981
- Genes for Growth Hormone, Chorionic Somatomammotropin, and Growth Hormone-Like Gene on Chromosome 17 in HumansScience, 1980
- Genes Coding for Histone Proteins in Man Are Located on the Distal End of the Long Arm of Chromosome 7Science, 1979
- The ovalbumin gene: Alleles created by mutations in the intervening sequences of the natural geneCell, 1979
- The isolation and characterization of linked δ- and β-globin genes from a cloned library of human DNACell, 1978
- Construction and analysis of recombinant DNA for human chorionic somatomammotropinNature, 1977
- Localization of the human α-globin structural gene to chromosome 16 in somatic cell hybrids by molecular hybridization assayCell, 1977
- The Status of the Gene Map of the Human ChromosomesScience, 1977
- Three abundance classes in HeLa cell messenger RNANature, 1974
- Functional Organization of Genetic Material as a Product of Molecular EvolutionNature, 1971