DECREASE OF NON-SUPPRESSIBLE INSULIN-LIKE ACTIVITY AFTER PANCREATECTOMY AND NORMALIZATION BY INSULIN THERAPY

Abstract

Non-suppressible insulin-like activity (NSILA-S) was determined in 5 dogs before and after pancreatectomy and again during insulin therapy. All NSILA-S determinations were carried out on serum samples which were passed over Sephadex G-50 columns equilibrated with 1 M acetic acid. The levels of NSILA-S decreased drastically shortly after pancreatectomy and rose slowly after institution of insulin therapy, to normal levels. During the period of severe diabetes after pancreatectomy the concentration of growth hormone [GH] was elevated. These findings indicate that: the pancreas cannot be the site of synthesis and release of NSILA-S; NSILA-S levels do not always parallel GH levels and the synthesis and secretion of NSILA-S among other factors is under the control of insulin.