Inactivation of Non‐A, Non‐B Virus Infectivity by a Beta Propiolactone/Ultraviolet Irradiation Treatment and Aerosil Adsorption Procedure Used for Preparation of a Stabilized Human Serum

Abstract

Inoculation of chimpanzees revealed that starting material for the preparation of a pooled stabilized human serum preparation was contaminated with non-A, non-B hepatitis virus(es). This material was subjected to .beta.-propiolactone/UV irradiation treatment and adsorption with an insolubilized silicic acid (Aerosil-380) under conditions employed for sterilization of the final product. The treated material did not transmit hepatitis to 2 inoculated chimpanzees. These animals were subsequently found to be susceptible to non-A, non-B infection by challenge with the untreated material. These findings demonstrate a non-A, non-B type hepatitis virus to be susceptible to inactivation by the methods employed for sterilization of this stabilized human serum preparation.