Polypeptide Synthesis in Sea Urchin Embryogenesis: An Examination with Synthetic Polyribonucleotides
- 10 May 1963
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 140 (3567), 664-666
- https://doi.org/10.1126/science.140.3567.664
Abstract
After fertilization of the sea urchin egg the rate of protein synthesis by a cell-free ribosomal system increases markedly. This increase can be attributed to newly synthesized messenger RNA, since (i) the rate of polypeptide synthesis elicited by synthetic messenger polyribonucleotides changes only slightly after fertilization, and (ii) the enzymes for the formation of amino acyl transfer RNA's of phenylalanine and leucine and the polymerization of polypeptide are in excess in the unfertilized egg. Polypeptide synthesis has been characterized in development to the gastrula stage.Keywords
This publication has 10 references indexed in Scilit:
- Interrelation of messenger polyribonucleotides and ribosomes in the sea urchin egg during embryonic developmentBiochemical and Biophysical Research Communications, 1962
- SYNTHETIC POLYNUCLEOTIDES AND THE AMINO ACID CODE, IVProceedings of the National Academy of Sciences, 1962
- Characteristics of the Utilization of Nucleosides by Embryos of Paracentrotus lividusJournal of Biological Chemistry, 1962
- AN INTERMEDIATE IN THE BIOSYNTHESIS OF POLYPHENYLALANINE DIRECTED BY SYNTHETIC TEMPLATE RNAProceedings of the National Academy of Sciences, 1962
- Activation of ribosomes in sea urchin eggs in response to fertilizationExperimental Cell Research, 1961
- A Simplified Procedure for the Preparation of Tyrosine- and Valine-Acceptor Fractions of Yeast “Soluble Ribonucleic Acid”Journal of Biological Chemistry, 1961
- PROLONGATION OF LIFE-SPAN OF SEA URCHIN SPERMATOZOA, AND IMPROVEMENT OF THE FERTILIZATION-REACTION, BY TREATMENT OF SPERMATOZOA AND EGGS WITH METAL-CHELATING AGENTS (AMINO ACIDS, VERSENE, DEDTC, OXINE, CUPRON)The Biological Bulletin, 1953
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- THE NUCLEIC ACIDS OF PLANT TISSUES .1. THE EXTRACTION AND ESTIMATION OF DESOXYPENTOSE NUCLEIC ACID AND PENTOSE NUCLEIC ACID1950
- The Determination of Enzyme Dissociation ConstantsJournal of the American Chemical Society, 1934