Purification and Properties of a Catechol Methyltransferase of the Yeast Candida tropicalis
Open Access
- 1 October 1979
- journal article
- research article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 34 (9-10), 709-714
- https://doi.org/10.1515/znc-1979-9-1010
Abstract
In an effort to investigate catechol methyltransferase activity in sources other than mammalian tissues and cells, a high level of enzyme activity was found in the yeast fungus C. tropicalis CBS 94. Partial purification of the enzyme (about 550-fold with a recovery of 7%) could be achieved by using ion-exchange and gel filtration techniques. The MW was estimated at 32,000 .+-. 2,000 by gel filtration on Sephadex G-100. In isoelectric focusing experiments on Sephadex G-75 the enzyme exhibited a pI-value of 5.0 .+-. 0.1. In contrast to catechol methyltransferase from various mammalian tissues, the enzyme activity was prepared from the pH 5-sediment. The substrate specificity is comparable to other catechol methyltransferases.Keywords
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