REGULATION OF THE IMMUNE RESPONSE
Open Access
- 1 March 1973
- journal article
- Published by Rockefeller University Press in The Journal of Experimental Medicine
- Vol. 137 (3), 721-739
- https://doi.org/10.1084/jem.137.3.721
Abstract
The specificity of antigen recognition by thymus-derived helper cells (T cells) and antibody was examined in mice, heterologous erythrocyte antigens from sheep (SRBC), goat (GRBC), burro (BRBC), chicken (CRBC), and toad (TRBC) being used. Antibody specificity was tested by a number of functional assays: hemagglutination, hemolysis, and immune suppression. The specificity of T cells was determined by titrating their ability to help the in vitro antitrinitrophenol (TNP) responses of mouse spleen cultures immunized with the hapten coupled to the various test erythrocytes as carrier. Anti-SRBC antibody cross-reacted with GRBC, but not with BRBC, CRBC, or TRBC. In contrast, SRBC-primed helper T cells cross-reacted with both GRBC and BRBC, but not with CRBC or TRBC, indicating a difference in the specificity of antigen recognition between the cellular and the humoral immune responses.Keywords
This publication has 36 references indexed in Scilit:
- Immunogenicity of Glucagon: Determinants Responsible for Antibody Binding and Lymphocyte StimulationScience, 1971
- Two distinct populations of peripheral lymphocytes in mice distinguishable by immunofluorescence.1970
- An in Vitro Primary Immune Response to 2, 4, 6-Trinitrophenyl Substituted Erythrocytes: Response against Carrier and HaptenThe Journal of Immunology, 1970
- The immunoglobulin nature of the antigen recognition site on cells mediating transplantation immunity and delayed hypersentivity.1970
- Antitrinitrophenyl (TNP) Plaque Assay. Primary Response of Balb/c Mice to Soluble and Particulate ImmunogenExperimental Biology and Medicine, 1969
- Specific Inactivation of Antigen-reactive Cells with 125I-Labelled AntigenNature, 1969
- Genetic Control of Specific Immune ResponsesAdvances in Immunology, 1969
- Antigenic Competition: Cellular or HumoralScience, 1967
- CELL POPULATIONS AND CELL PROLIFERATION IN THE IN VITRO RESPONSE OF NORMAL MOUSE SPLEEN TO HETEROLOGOUS ERYTHROCYTESThe Journal of Experimental Medicine, 1967
- IMMUNIZATION OF DISSOCIATED SPLEEN CELL CULTURES FROM NORMAL MICEThe Journal of Experimental Medicine, 1967