The Influence of Calcium Ions on the Preparation of the (Ca2++ Mg2+)-Activated Membrane ATPase in Human Red Cells
- 1 January 1972
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 30 (3), 313-320
- https://doi.org/10.3109/00365517209084296
Abstract
Membranes were prepared from red cells both in the presence of 0.4–0.5 mmol/l Ca2+ and without Ca2+. The Ca2+-stimulated ATPase of membranes prepared without Ca2+ showed reduced specific activity, decreased affinity towards Ca2+, and a pH optimum of 7.40 compared to a pH optimum of 7.15 of the Ca2+-stimulated ATPase of Ca2+-treated membranes. The phenomena are possibly due to the formation of a modified state of the ATPase in the membranes prepared without Ca2+.Keywords
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