Concanavalin A induces microtubule assembly and specific granule discharge in human polymorphonuclear leukocytes.
Open Access
- 1 March 1976
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 68 (3), 781-787
- https://doi.org/10.1083/jcb.68.3.781
Abstract
Human neutrophils stimulated by concanavalin A (Con A, 100 microng/ml) contained markedly enhanced numbers of microtubules and discharged peroxidase-negative (specific) but not peroxidase-position (azurophile) granules. Release of lysozyme from specific granules was dose and time dependent, could be inhibitied by alpha-methyl-D-mannoside, and enhanced by cytochalasin B. Many microtubules were associated with internalized plasma membrane bearing Con A binding sites.Keywords
This publication has 16 references indexed in Scilit:
- Mechanisms of lysosomal enzyme release from human leucocytesClinical Immunology and Immunopathology, 1974
- Calcium-induced lysozyme secretion from human polymorphonuclear leukocytesBiochemical and Biophysical Research Communications, 1974
- Selective labilization of specific granules in polymorphonuclear leukocytes by phorbol myristate acetate.1974
- Effects of Phagocytosis and Colchicine on the Distribution of Lectin-Binding Sites on Cell SurfacesProceedings of the National Academy of Sciences, 1974
- Mechanisms of Lysosomal Enzyme Release from Human Leukocytes II. EFFECTS OF cAMP AND cGMP, AUTONOMIC AGONISTS, AND AGENTS WHICH AFFECT MICROTUBULE FUNCTIONJournal of Clinical Investigation, 1974
- Mechanisms of Lysosomal Enzyme Release from Human Leukocytes: Microtubule Assembly and Membrane Fusion Induced by a Component of ComplementProceedings of the National Academy of Sciences, 1973
- MicrotubulesAnnual Review of Biochemistry, 1973
- The inhibition of phagocytosis and facilitation of exocytosis in rabbit polymorphonuclear leukocytes by cytochalasin B.1973
- THE DEVELOPMENT OF NEUTROPHILIC POLYMORPHONUCLEAR LEUKOCYTES IN HUMAN BONE MARROWThe Journal of Experimental Medicine, 1971
- Metalloenzymes and myocardial infarction. II. Malic and lactic dehydrogenase activities and zinc concentrations in serum.1956