DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
- 1 March 1979
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 76 (3), 1373-1376
- https://doi.org/10.1073/pnas.76.3.1373
Abstract
The feasibility of transforming mouse cells deficient in adenine phosphoribosyltransferase (aprt; AMP:pyrophosphate phosphoribosyltransferase, EC 2.4.2.7) to the aprt+ phenotype by DNA-mediated gene transfer was demonstrated. Transformation was effected by using unfractionated high MW genomic DNA from Chinese hamster, human and mouse cells and restriction endonuclease-digested DNA from rabbit liver. The transformation frequency observed was 1-10 colonies/106 cells per 20 .mu.g of donor DNA. Transformants displayed enzymatic activity that was donor derived as demonstrated by isoelectric focusing of cytoplasmic extracts. These transformants fall into 2 classes: those that are phenotypically stable when grown in the absence of selective pressure and those that are phenotypically unstable under the same conditions.This publication has 14 references indexed in Scilit:
- Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donorCell, 1978
- Transformation of yeast.Proceedings of the National Academy of Sciences, 1978
- Transfer of purified herpes virus thymidine kinase gene to cultured mouse cellsCell, 1977
- Mutant alleles for hypoxanthine phosphoribosyltransferase: Codominant expression, complementation, and segregation in hybrid Chinese hamster cellsSomatic Cell and Molecular Genetics, 1976
- Isolation and partial characterization of three methotrexate-resistant phenotypes from Chinese hamster ovary cellsSomatic Cell and Molecular Genetics, 1976
- Cotransfer of two linked human genes into cultured mouse cells.Proceedings of the National Academy of Sciences of the United States of America, 1976
- Transfer of the human gene for hypoxanthine-guanine phosphoribosyltransferase via isolated human metaphase chromosomes into mouse L-cells.Proceedings of the National Academy of Sciences of the United States of America, 1975
- Mutations affecting adenine phosphoribosyl transferase activity in Chinese hamster cellsCell, 1974
- Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran.1968
- Deletion of thymidine kinase activity from L cells resistant to bromodeoxyuridineExperimental Cell Research, 1963