Albuminated Polymer Surfaces for Biomedical Application

Abstract

Amino groups were added to the surfaces of Celgard 2400 membranes by exposing them to an ammonia plasma. The presence of amino groups on the surfaces was detected by an attenuated total reflection Fourier transform IR spectrometer and by the Auger electron spectrometer. Through these amino groups, [human bovine] albumin was attached to the membranes. In some experiments, the attached albumin was further stabilized by cross-linking with glutaraldehyde. The effect of washing the albuminated membranes with saline and with plasma was investigated. After the initial washout of albumin, the concentration of attached albumin tended to level off. The amount of albumin retained on the membranes varied between 275-357 .mu.g/cm2.