Synthesis of ribosomal proteins in merodiploid strains and in minicells ofEscherichia coli

Abstract

Merodiploid strains ofEscherichia coli containing episomes which carry one or several of the ribosomal protein (r-protein) transcriptional units were analysed to see whether the increase in the number of gene copies leads to an increased synthesis of the respective r-proteins. It was found that the amount of ribosomal proteins was (with the only exception of ribosomal protein S20) independent of the number of gene copies present. The comparison of the in vivo stability of r-proteins in haploid and merodiploid strains did not, within the time resolution of the experiment, provide any evidence for an increased rate of degradation of those proteins coded by more than one gene copy. These results indicate a tight coupling between the amount of ribosomal proteins synthesized and the level required irrespective of the number of gene copies present. With the aid of minicells from a strain containing the episome F'101 which carries thethr-leu segment of the chromosome it was demonstrated that (i) in vivo synthesis of r-protein S20 could proceed in the absence of the synthesis of ribosomal RNA and of other r-proteins, and (ii) r-protein S20 was degraded under conditions where it was not assembled into ribosomes.