PHASE-1 STUDY OF HIGH-DOSES OF AMINOPTERIN WITH LEUCOVORIN RESCUE IN PATIENTS WITH ADVANCED METASTATIC TUMORS

Abstract

A Phase 1 study was conducted of aminopterin (AMT) with leucovorin (LV) in 17 patients. AMT was administered by bolus injection every 7-14 days in dosages from 25-425 mg/m2. LV rescue was instituted at 24 h and continued for 48-72 h. At dosages above 50 mg/m2, nephrotoxicity defined as greater than or equal to a 25% increase in serum creatinine 24 h after AMT administration was observed, but its incidence was not strictly dose related. Urinary alkalinization and volume expansion appeared to reduce the incidence of nephrotoxicity. Nephrotoxic drug courses were associated with 24 h plasma AMT levels [3.6 .+-. 2.0 (SD) .times. 10-6 M] which were significantly higher than nonnephrotoxic courses (1.6 .+-. 1.0 .times. 10-6 M) (P < 0.05). In nonnephrotoxic courses, serum elimination pharmacokinetics appeared to be biphasic with a t1/2 .alpha. [.alpha.-phase half life] of 1.08 .+-. 0.01 h and t1/2 .beta. [.beta.-phase half life] of 12.31 .+-. 0.06 h. Systemic toxicity (myelosuppression and mucositis) could be prevented in patients with impaired AMT clearance by the administration of LV at an increased dose rate. In several courses, systemic toxicity occurred in spite of apparently normal plasma clearance, suggesting that 24 h plasma levels may not accurately reflect intracellular drug effects. Cytokinetic studied on bone marrow aspirates allowed determination of the rescue effect of LV and may prove useful in predicting marrow protection.