HPLC—32P-postlabelling analysis of 1,N6-ethenodeoxyadenosine and 3,N4-ethenodeoxycytidine
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Mutagenesis
- Vol. 4 (1), 75-77
- https://doi.org/10.1093/mutage/4.1.75
Abstract
A 32P-postlabelling procedure coupled with HPLC has been developed to detect and measure the cyclic nucleic acid adducts 1,N6-ethenodeoxyadenosine and 3,N4-ethenodeoxycytidine in DNA. Chloroacetaldehyde-modified DNA containing these adducts was enzymatically digested to 3'-monophosphates and adducts were separated by ion-pair reverse-phase HPLC on PL RP-S prior to 32P-postlabelling with carrier free [γ-32P]ATP. Following 3'-dephosphorylation with nuclease PI the resulting [5'-32P] monophosphate adducts were finally resolved by HPLC on PL RP-S and assayed by liquid scintillation counting.This publication has 9 references indexed in Scilit:
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