Metabolites of vitamin D in normal and X-linked hypophosphatemic mice
- 1 December 1984
- journal article
- research article
- Published by Springer Nature in Calcified Tissue International
- Vol. 36 (1), 662-667
- https://doi.org/10.1007/bf02405387
Abstract
Hyp mice are a model for human X-linked hypophosphatemia (vitamin D-resistant rickets.) To determine whether an abnormality of vitamin D metabolism exists in this disease, the profiles of the metabolites of vitamin D were determined in normal andHyp mouse plasma.Hyp and normal mice were fed a vitamin D-deficient diet and received 1,23H-vitamin D3 at 16 Ci/mmol by stomach tube at 5 ng/g body weight (0.21 µCi/g b.w.) on alternate days for 14 days. The dose of vitamin D given maintained near normal plasma 25-OH-vitamin D. Thus the mice were in a vitamin D-replete state with all metabolite pools labeled with3H. Plasma was collected from 4 normal and 4Hyp mice. The plasma was extracted, and the extracts were chromatographed separately for each mouse on an LH-20 column. Each major peak of radioactivity was rechromatographed using high performance liquid chromatography on a Zorbax-Sil column using solvent systems known to resolve several vitamin D metabolites. Twenty-one radioactive peaks were identified. The disintegrations per minute of3H in each peak were quantified and converted to plasma concentration using the known specific activity of the administered vitamin D. The 25-OH-vitamin D accounted for 55% of the circulating radioactivity, and 24,25-(OH)2-vitamin D accounted for 22%. The plasma levels of 24,25-(OH)2-vitamin D were similar to levels previously reported by us using protein binding assays. No peaks of radioactivity were missing in the plasma extracts of theHyp mice. Also there was no evidence that plasma 24,25-(OH)2-vitamin D was elevated in theHyp mice.Keywords
This publication has 20 references indexed in Scilit:
- Serum 1,25-dihydroxyvitamin D concentration in hypophosphatemic vitamin D-resistant ricketsCalcified Tissue International, 1981
- Increased urinary excretion of cyclic nucleotides in X-linked hypophosphatemic (Hyp) miceCellular and Molecular Life Sciences, 1981
- Evaluation of a Role for 1,25-Dihydroxyvitamin D3 in the Pathogenesis and Treatment of X-linked Hypophosphatemic Rickets and OsteomalaciaJCI Insight, 1980
- Osteomalacia and altered magnesium metabolism in the X-linked hypophosphatemic mouseCalcified Tissue International, 1979
- Serum 1,25-Dihydroxyvitamin D Levels in Normal Subjects and in Patients with Hereditary Rickets or Bone DiseaseNew England Journal of Medicine, 1978
- Effect of dietary calcium and phosphorus on intestinal calcium absorption and vitamin D metabolismArchives of Biochemistry and Biophysics, 1978
- Influence of Phosphate Depletion on the Biosynthesis and Circulating Level of 1α,25-Dihydroxyvitamin DPublished by Springer Nature ,1977
- Metabolism of Vitamin D3-3H in Vitamin D-Resistant Rickets and Familial Hypophosphatemia*JCI Insight, 1967
- A new method for determining micro quantities of calcium in biological materialsAnalytical Biochemistry, 1967
- Metabolism of tritiated vitamin D3 in familial vitamin D-resistant rickets with hypophosphatemiaThe Journal of Pediatrics, 1967