Functional properties of nicotinic acetylcholine receptor subunits expressed in various combinations
- 20 April 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 214 (2), 253-258
- https://doi.org/10.1016/0014-5793(87)80065-0
Abstract
The four kinds of subunits of the Torpedo californica nicotinic acetylcholine receptor have been produced in various combinations by injecting Xenopus oocytes with the corresponding subunit-specific mRNAs synthesized by transcription in vitro of the cloned cDNAs. Functional analysis suggests that association of the alpha-subunit with either the gamma- or the delta-subunit is a prerequisite for generating the conformation necessary for agonist binding. The acetylcholine receptor devoid of either the beta-, gamma- or delta-subunit exhibits weak channel activity.Keywords
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