The Measurement of Leucine Enkephalinat the Femtomole Level by Fast Atom Bombardment Mass Spectrometry/Mass Spectrometry Methods
- 1 January 1986
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 19 (19-20), 1963-1971
- https://doi.org/10.1080/00032718608064539
Abstract
FAB-MS/MS methods are used to quantify the neuropeptide leucine enkephalin (LE = YGGFL) in synthetic solutions. Maximum molecular specificity is provided by monitoring two metastable transitions from the LE (M + H)+, 556 → 425 and 556 → 336, in a forward geometry (E, B) mass spectrometer using a B/E linked-field selected reaction monitoring technique. Obtained sensitivity is 40 pg LE, which equals 72 fmol. The statistics of the best-fit straight lines are, for m/z 425: y = 34 + 166 (r = 0.999), and for m/z 336: y = 2.5x + 17.2 (r = 0.996).Keywords
This publication has 5 references indexed in Scilit:
- Positive and negative fast-atom bombardment—collision-activated dissociation—linked-field scanned mass spectra of leucine enkephalinInternational Journal of Mass Spectrometry and Ion Processes, 1983
- Preparation of stable isotope-incorporated peptide internal standards for field desorption mass spectrometry quantification of peptides in biologic tissueJournal of Mass Spectrometry, 1983
- Measurement of leucine enkephalin in caudate nucleus tissue with fast atom bombardment-collision activated dissociation-linked field scanning mass spectrometryJournal of Mass Spectrometry, 1983
- Fast atom bombardment study of glycerol: mass spectra and radiation chemistryThe Journal of Physical Chemistry, 1982
- Fast atom bombardment mass spectrometry of bradykinin and related oligopeptidesJournal of Mass Spectrometry, 1981