Improved conditions for murine epidermal cell culture
- 1 February 1980
- journal article
- research article
- Published by Springer Nature in In Vitro Cellular & Developmental Biology - Plant
- Vol. 16 (2), 180-188
- https://doi.org/10.1007/bf02831508
Abstract
An improved method for cultivating newborn mouse epidermal cells has been developed that increases the longevity, epithelial nature and efficiency of cell-line establishment. The use of Super Medium, an enriched Waymouth's formulation, increased proliferation for long periods of time, as did incubation at 31°C rather than 37°C. The fetal bovine serum requirement was found to be reduced at the lower temperature. An increase in labeling indices was seen when epidermal growth factor (EGF) or the cyclic nucleotides were added and the presence of EGF receptors was determined. Of the prostaglandins (PG) examined, PGE1 and PGE2 produced the greatest increase in DNA synthesis. The PG precursors, arachidonic and 8,11,14-eicosatrienoic acid, were also greatly stimulatory. The use of a lethally irradiated 3T3 feeder layer at 31°C proved superior in maintenance of an epithelial morphology. Subculturable cell lines were established much more readily and reproducibly in carcinogen-treated cultures grown under the improved conditions.This publication has 22 references indexed in Scilit:
- Loss of Division Potential in Vitro: Aging or Differentiation?Science, 1978
- Stratification, specialization, and proliferation of primary keratinocyte cultures. Evidence of a functioning in vitro epidermal cell system.The Journal of cell biology, 1978
- Retinyl acetate modulation of cell growth kinetics and carcinogen-cellular interaction in mouse epidermal cell culturesChemico-Biological Interactions, 1977
- Cyclic Gmp, Cyclic Amp, And The Yin Yang Hypothesis Of Biologic RegulationJournal of Investigative Dermatology, 1976
- The relationship between concentration of prostaglandin E and rates of cell replicationExperimental Cell Research, 1974
- Increased cyclic GMP and decreased cyclic AMP levels in the hyperplastic, abnormally differentiated epidermis of psoriasisLife Sciences, 1973
- Studies of Proliferative Capabilities in Isolated Epidermal Basal and Differentiated CellsJournal of Investigative Dermatology, 1971
- The Growth of Fetal Mouse Skin in Cell Culture and Transplantation to F1 Mice**From the Experimental Pathology Branch, National Cancer InstituLe, Bethesda, Maryland 20014.Journal of Investigative Dermatology, 1970
- Tissue interactions and cytodifferentiationJournal of Experimental Zoology, 1964
- The Stimulation of Epidermal Keratinization by a Protein Isolated from the Submaxillary Gland of the Mouse**From the Department of Biochemistry and the Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee.Journal of Investigative Dermatology, 1963