A partial purification and some properties of the cytochrome oxidase from pig-heart muscle
- 28 February 1963
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 86 (3), 535-540
- https://doi.org/10.1042/bj0860535
Abstract
A method for the solubilization and fractionation of pig-heart particles for the isolation of cytochrome oxidase is described. Analysis of five preparations for iron and copper has shown an almost 11 ratio, although this ratio was occasionally 1:2. The spectra of the pyridine haemochromogens obtainable from the preparation are described. The spectral properties of the preparation alone, and in combination with ligands, are described. The spectrum obtained on mixing oxygen with the reduced preparation in the presence of an excess of dithionite is described.Keywords
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