CYTOPLASMIC AGGREGATES IN D-GALACTOSAMINE INDUCED LIVER-INJURY

Abstract

D-galactosamine treatment leads to the formation of PAS[periodic acid Schiff]-positive granules or aggregates in the cytoplasm of mouse liver cells. The granules consist of particles surround by membranes of rough endoplasmic reticulum. Part of the particles is pronase-sensitive and amylase resistant, staining positively by the Thiery silver proteinate method. The other part is stained positively by EDTA preferentional staining. According to the cyto- and histochemical results the granules consist of ribosomes and abnormal basic glycogen. The aggregates are removed from the cytoplasm mostly by lysosomal degradation.