Binding of the N‐terminal 63 kDa portion of connectin/titin to α‐actinin as revealed by the yeast two‐hybrid system
Open Access
- 13 January 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 401 (1), 65-67
- https://doi.org/10.1016/s0014-5793(96)01432-9
Abstract
Connectin/titin is a 3000 kDa protein which links the myosin filament to the Z‐line in vertebrate striated muscle sarcomeres. To search for the Z‐line proteins to which connectin binds, the yeast two‐hybrid system was applied using cDNA coding the N‐terminal 63 kDa fragment of connectin. Two clones coding the C‐terminal half region of α‐actinin (amino acids, 343–897 and 446–897) were obtained. Enzyme‐linked immunosorbent assay clearly demonstrated the interactions of α‐actinin and the N‐terminal 63 kDa fragment of connectin in vitro. Thus it is concluded that the N‐terminal 63 kDa portion of connectin binds to α‐actinin in the Z‐line of myofibrillar sarcomeres.Keywords
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