The Rapid Determination of Neopterine in Human Urine by Isocratic High-Performance Liquid Chromatography

Abstract

An isocratic high-performance liquid chromatography method is described for the determination of neopterine [2-amino-4-hydroxy-6-trihydroxypropylpteridine] eliminated in human urine, using a .mu.-Bondapak C18 column (300 .times. 3.9 mm I.D.) and a strongly polar phosphate buffer (pH 6.2) for elution. This analysis requires only 15 min and allows very good reproducibility of retention times. Times method is well suited for automation and routine clinical laboratory to quantify human urinary neopterine in healthy subjects and in subjects with malignant disorders.