Complete sequence and structure of the gene for human adenosine deaminase
- 16 December 1986
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 25 (25), 8234-8244
- https://doi.org/10.1021/bi00373a017
Abstract
The nucleotide sequence of the human adenosine deaminase gene was determined. The gene was isolated in a series of overlapping .lambda. phage clones containing human germ line DNA. A total of 36741 base pairs were sequenced, including 32,040 base pairs from the transcription initiation site to the polyadenylation site, 3935 base pairs of 5''-flanking DNA, and 766 base pairs of 3''-flanking DNA. The gene contains 12 exons separated by 11 introns. The exons range in size from 62 to 325 base pairs while the introns are 76-15 166 base pairs in size. The area sequenced contains 23 copies of Alu repetitive DNA and a single copy of an "O" family repeat. All but one of these repeat sequences are located in the first three introns or the 5''-flanking region. The apparent promoter region of the gene lacks the "TATA and "CAAT" sequences often found in eucaryotic promoters and is extremely G/C rich. Contained within this region are areas homologous to other G/C-rich promoters, including six decanucleotide sequences that are highly homologous to sequences identified as functional binding sites for transcription factor Sp1.This publication has 19 references indexed in Scilit:
- HMG CoA reductase: A negatively regulated gene with unusual promoter and 5′ untranslated regionsCell, 1984
- Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene.Proceedings of the National Academy of Sciences, 1984
- Cloning of cDNA sequences of human adenosine deaminase.Proceedings of the National Academy of Sciences, 1983
- Lambda replacement vectors carrying polylinker sequencesJournal of Molecular Biology, 1983
- Relationship between the total size of exons and introns in protein-coding genes of higher eukaryotes.Proceedings of the National Academy of Sciences, 1982
- Structure of amplified normal and variant dihydrofolate reductase genes in mouse sarcoma S180 cells.Journal of Biological Chemistry, 1982
- Purification, characterization and radioimmunoassay of adenosine deaminase from human leukaemic granulocytesBiochemical Journal, 1981
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- Human adenosine deaminase. Purification and subunit structure.Journal of Biological Chemistry, 1977
- Purification of human erythrocyte adenosine deaminase by affinity column chromatography.Journal of Biological Chemistry, 1976