Light-stimulated phosphorylation of rhodopsin in the retina: the presence of a protein kinase that is specific for photobleached rhodopsin.
- 1 January 1975
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 72 (1), 381-385
- https://doi.org/10.1073/pnas.72.1.381
Abstract
A protein kinase has been extracted from bovine rod outer segments by a mild procedure. The enzyme acts specifically on photobleached, not unbleached, rhodopsin and will not catalyze the phosphorylation of histones, phosvitin, or casein. We propose the name "opsin kinase" for the enzyme, which is not affected by cyclic nucleotides but which is inhibited by theophylline. Preparations of purified rod outer segments, however, appear to contain only low concentration of opsin phosphatase activity.Keywords
This publication has 24 references indexed in Scilit:
- Rhodopsin and light-sensitive kinase activity of retinal outer segmentsExperimental Eye Research, 1974
- Physiology and enzymology of frog photoreceptor membranesExperimental Eye Research, 1974
- Light-regulated guanosine 3′, 5′-monophosphate phosphodiesterase of bovine retinaNature, 1974
- Guanylate Cyclase: Inhibition by Light in Retinal PhotoreceptorsScience, 1974
- Phosphorylation of rhodopsin in bovine photoreceptor membranes. Dark reaction after illuminationBiochemistry, 1973
- Light dependent phosphorylation of rhodopsin by ATPFEBS Letters, 1972
- Dopamine-Sensitive Adenyl Cyclase: Possible Role in Synaptic TransmissionScience, 1971
- Proteins of bovine retinal outer segments: Electrophoresis on polyacrylamide gels in the presence of sodium dodecyl sulfateFEBS Letters, 1971
- Scintillation counting of 32P without added scintillator in aqueous solutions and organic solvents and on dry chromatographic mediaAnalytical Biochemistry, 1970
- Stimulation by Cyclic AMP of Intrinsic Protein Kinase Activity in Ox Brain Membrane PreparationsNature, 1970