Use of the Yolk Sac of the Developing Chicken Embryo in the Isolation of the Agent ofLymphogranuloma Venereum

Abstract

Two strains of the agent of lymphogranuloma venereum were isolated by inoculation of suspensions of pus from human inguinal buboes directly into the yolk sacs of embryonated chicken eggs. The possibility that supposed strains of lymphogranuloma venereum isolated in the past by animal passage may have represented related agents harbored in the passage animals in a latent state would not seem to arise in the present case inasmuch as such agents have never been found as spontaneous in-fectors in the fertile chicken egg. In all ways, and particularly in sulfonamide susceptibility, tissue tropisms, and type of endotoxin produced, the 2 strains resemble others of lymphogranuloma venereum; in the 3 latter characteristics they differ from all other members of the lympho-granuloma-trachoma-psittacosis group.