Cloning and characterization of human and mouse homologs of the Drosophila calcium-activated potassium channel gene, slowpoke

Abstract

Potassium channels play Important roles in a wide variety of physiological processes. Although several genes encoding voltage-activated potassium channels have been analyzed at the molecular level, no calcium-activated potassium channel gene has yet been characterized In humans. In an effort to provide the foundation for functional analysis of such polypeptides we report the cloning of mouse and human homologs of the Drosophlla melanogaster calcium-activated potassium channel gene, slowpoke. Both the human and mouse genes encode polypeptides that have more than 50% amlno acid Identities with their Drosophlla counterpart. In addition, like the Drosophlla slowpoke gene, both the mouse and human genes generate multiple transcripts by alternative splicing. The human gene maps to chromosome 10 based on the results of polymerase chain reaction analysis of genomlc DNA from human-hamster hybrid cell lines. Because calcium-activated potassium channels participate In wide variety of cellular functions including neuro-muscular communication, secretion and cellular Immunity, their continued analysis promises to have broad biological and medical significance.