Molecular cloning of fragments of bacteriophage T4 DNA

Abstract

Non-glucosylated T4 DNA was digested with R. EcoRI and the resulting fragments covalently joined to λ vectors. The genetic content of each λ-T4 hybrid was determined by marker-rescue tests. The isolation of many recombinants containing partialdigestion products of T4 DNA provided the overlapping sequences necessary to order fragments within the T4 genome. The present analyses include parts of the “early” region between genes 42 and 46, and much of the “late” region between genes 50 and 29. T4 cytosine-DNA digested to completion by R.EcoRI was used to identify the fragments of DNA within the λ-T4 recombinants. The T4 cytosine-DNA was also sensitive to R.HindIII and R.Xho but not to R.BamH1.