Molecular structure of mammalian neuropeptide Y: analysis by molecular cloning and computer-aided comparison with crystal structure of avian homologue.
- 1 April 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (8), 2532-2536
- https://doi.org/10.1073/pnas.84.8.2532
Abstract
Identification and characterization of the cDNA encoding rat neuropeptide Y revealed the nucleotide sequence coding for a 98-amino acid precursor. The deduced amino acid sequence for rat neuropeptide Y is identical to the human peptide and is highly homologous to avian pancreatic polypeptide. The tertiary structure of avian pancreatic polypeptide has been previously derived from crystallographic data by Blundell and coworkers. The homology between neuropeptide Y and avian pancreatic polypeptide preserves all of the residues essential for the maintenance of the tertiary structure. Thus, it has been possible to compute a three-dimensional model of the mammalian neuropeptide, neuropeptide Y, based on the known structure of the avian homologue. This model suggests that neuropeptide preserves a compact tertiary structure characterized by extensive hydrophobic interactions between an N-terminal polyproline-II-like helix and a C-terminal .alpha.-helix. The model has been used to identify amino acids residing in key positions within this structure and, thereby, to direct future analysis of neuropeptide Y structure-function relationships.This publication has 32 references indexed in Scilit:
- Prediction of the folding of short polypeptide segments by uniform conformational samplingBiopolymers, 1987
- Localisation of neuropeptide Y in nerves of the rat cardiovascular system and the effect of 6-hydroxydopamineCardiovascular Research, 1985
- Neuroreptide Y: Stimulation of feeding and drinking by injection into the paraventricular nucleusLife Sciences, 1984
- An analysis of incorrectly folded protein modelsJournal of Molecular Biology, 1984
- Neuropeptide Y Distribution in the Rat BrainScience, 1983
- Eukaryotic ribosomes can recognize preproinsulin initiation codons irrespective of their position relative to the 5′ end of mRNANature, 1982
- Peptidergic neuronesNature, 1980
- The protein data bank: A computer-based archival file for macromolecular structuresJournal of Molecular Biology, 1977
- Screening λgt Recombinant Clones by Hybridization to Single Plaques in SituScience, 1977
- The interpretation of protein structures: Estimation of static accessibilityJournal of Molecular Biology, 1971