STUDIES WITH STAPHYLOCOCCAL TOXINS: III. THE APPLICATION OF PAPER IONOPHORESIS TO THE RESOLUTION OF COMPONENTS OF TOXIC CONCENTRATES
- 1 June 1955
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Microbiology
- Vol. 1 (6), 401-411
- https://doi.org/10.1139/m55-051
Abstract
A substance present in culture filtrates of enterotoxigenic staphylococci that is distinct from alpha-, beta-, or delta-lysins causes vomiting; in cats when injected by the intraperitoneal route. An emetic fraction containing only vestigial traces of lysins can be resolved into three protein components and one polysaccharide by use of the Durrum ionophoresis method. The only component of this group that gave indications of enterotoxicity was a protein with specific mobility, relative to the others. Hemolytic proteins were separated by paper ionophoresis using the method of McKinley, Maw, Oliver, and Common.Keywords
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