Enhancement of Infectivity of Encephalomyocarditis Virus RNA by Amphotericin B Methyl Ester
- 1 February 1979
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 42 (2), 297-303
- https://doi.org/10.1099/0022-1317-42-2-297
Abstract
The methyl ester of amphotericin B (AmBME), a macrolide polyene antibiotic, enhanced the infectivity of encephalomyocarditis (EMC) virus RNA for L929 cells. AmBME alone (100 µg/ml) resulted in increases in EMC virus RNA infectivity of 10- to 100-fold. Addition of DEAE dextran at concentrations (5 µg/ml), which alone slightly suppressed EMC virus RNA infectivity, further augmented the effects of AmBME (augmentation in infectivity up to 750-fold). AmBME did not inhibit RNase, did not enhance EMC virus infectivity and increased infectivity of EMC virus RNA which was already cell-associated. The polyenes are probably acting by increasing intracellular penetration of polyribonucleotides.This publication has 1 reference indexed in Scilit:
- Increase by Calcium in Production of Interferon by L929 Cells Induced with Polyriboinosinate-Polyribocytidylate ComplexJournal of General Virology, 1978