Inhibition of cellular response to platelet-derived growth factor by lowMrphosphotyrosine protein phosphatase overexpression
- 25 July 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 349 (1), 7-12
- https://doi.org/10.1016/0014-5793(94)00620-2
Abstract
The role of low M r phosphotyrosine protein phosphatase (PTPase) in the control of cell proliferation was studied. A synthetic gene coding for PTPase was transfected and expressed in NIH/3T3 fibroblasts. The effects of the enzyme were particularly evident when cells were stimulated by platelet-derived growth factor (PDGF). The mitogenic response to PDGF was decreased and the inhibition reached 90%. This effect was more pronounced with respect to fetal calf serum stimulation. Hormone-dependent autophosphorylation of the PDGF receptor was significantly reduced. These results demonstrate that low M r PTPase, a cytosolic enzyme, not only affects cellular response to PDGF but also reduces the membrane receptor autophosphorylation.Keywords
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