Comparison of Results by Four Different Procedures for Determination of 17-Hydroxycorticosteroids

Abstract

A Porter and Silber procedure, a fluorometric procedure, a double-isotope derivative procedure, and a competitive protein-binding assay procedure were used to determine 17-hydroxycorticosteroids in plasma from normal individuals and from patients with endocrinopathies. Results from each of these procedures were intercompared. Results of the fluorometric procedure and the competitive protein-binding assay compared favorably with results obtained by the more elaborate and difficult double-isotope derivative method. The Porter and Silber method is less specific. This study should be a useful aid in comparing values with those of other laboratories and in selecting the most advantageous method for use by a particular laboratory.