Bile protein secretion in the rat stimulated by taurocholate effect of chloroquine
- 1 June 1988
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Physiology and Pharmacology
- Vol. 66 (6), 749-753
- https://doi.org/10.1139/y88-119
Abstract
The biliary protein excretion during sodium taurocholate induced choleresis was studied in normal rats and in rats treated with the lysosomotropic agent, chloroquine. The analysis of the protein component in bile was made on SDS–polyacrilamide gel, and the individual polypeptides were quantitated by densitometry. The excretion of bile polypeptides was compared with that of lysosomal acid phosphatase. The biliary excretion of polypeptides of molecular mass lower than and equal to 54 kDa was markedly stimulated by taurocholate-induced choleresis. Chloroquine treatment of rats diminished the biliary excretion of such polypeptides and also inhibited their excretion induced by taurocholate. The behaviour of these polypeptides was well correlated to that of the lysosomal marker. The biliary excretion of polypeptide bands of a higher molecular mass (up to 140 kDa) did not show major changes during taurocholate-induced choleresis in any of the groups. The results indicate that biliary excretion of proteins in the rat may be either stimulated by taurocholate or may be independent of the bile salt. The former requires the functional integrity of chloroquine-sensitive hepatocyte compartments, which may involve the lysosomes.This publication has 19 references indexed in Scilit:
- Biliary protein output by isolated perfused rat livers. Effects of bile saltsBiochemical Journal, 1983
- The mechanism of biliary excretion of α1-acid glycoprotein in the rat: Evidence for a molecular weight-dependent, nonreceptor-mediated pathwayHepatology, 1982
- Enzymes and proteins in bile Variations in output in rat cannula bile during and after depletion of the bile-salt poolBiochemical Journal, 1981
- INFLUENCE OF COLCHICINE AND PHALLOIDIN ON BILE SECRETION AND HEPATIC ULTRASTRUCTURE IN THE RAT - POSSIBLE INTERACTION BETWEEN MICROTUBULES AND MICROFILAMENTS1980
- Coordinate Secretion of Acid Hydrolases in Rat BileJCI Insight, 1979
- Membranes and bile formation. Composition of several mammalian biles and their membrane-damaging propertiesBiochemical Journal, 1979
- Sources of the proteins of rat bileBiochimica et Biophysica Acta (BBA) - General Subjects, 1978
- Protein composition of rat bileCanadian Journal of Biochemistry, 1978
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- DETERMINATION OF SERUM PROTEINS BY MEANS OF THE BIURET REACTIONJournal of Biological Chemistry, 1949